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锤头核酶的分子生物学机制

来源:解剖科学进展 作者:郭晓华 2004-9-29
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摘要: 摘要:近二十年来,锤头核酶作为基因治疗的一种重要RNA酶类越来越受到人们的广泛关注,有关锤头核酶抗病毒作用机理及应用等问题的研究正在逐步深入。本文仅就其分子结构、催化功能及影响核酶作用功效因素等分子生物学机制的研究进展作一综述。 The structure, function and application of the hammerhead ribozyme。 Se......


  摘 要:近二十年来,锤头核酶作为基因治疗的一种重要RNA酶类越来越受到人们的广泛关注,有关锤头核酶抗病毒作用机理及应用等问题的研究正在逐步深入。本文仅就其分子结构、催化功能及影响核酶作用功效因素等分子生物学机制的研究进展作一综述。参考文献:

  [1]Birikh KR, Heaton PA, Eckstein F. The structure, function and application of the hammerhead ribozyme. Eur J Biochem, 1997, 245: 1

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  [14]Tuschl T, Gohlke C, Jovin TM. A Three-dimensional model for the hammerhead ribozyme based on fluorescence measurements. Science, 1994, 266: 785

  [15]Burgin AB, Gonzalez Jr C, Karpeisky AM, et al. Chemically modified hammerhead rihozymes with improved catalytic rates. Biochemistry, 1996, 35: 14090

  [16]Kuimelis RG, McLaughlin LW. Probing the cleavage activity of the hammerhead ribozyme using analog complexes. Nuc Aci Mol biol, 1996a 10: 197

  [17]McKay DB. Structure and function of the hammerhead ribozyme an unfinished story. RNA, 1996, 2: 395

  [18]Beigelman L, McSwiggen JA. Draper KG, et al. Chemical modification of hammerhead ribozyme., catalytic activity and nuclease resistance. J Biol Chem, 1995, 270: 25702

  [19]Bassi GS, Murchie AIH, Lilley DMJ. The ion-induced folding of the hammerhead ribozyme: core sequence changes that preturb folding into the inactive conformation. RNA, 1996, 2: 756

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